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Product Info Summary
| SKU: | M03713 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TCL1 Monoclonal Antibody
SKU/Catalog Number
M03713
BM5744 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TCL1 Monoclonal Antibody catalog # M03713. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCL1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03713)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFFA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TCL1 Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival.
Reactive Species
M03713 is reactive to TCL1A in Human
Observed Molecular Weight
13 kDa
Calculated molecular weight
13.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03713 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:100
Positive Control
WB: Ramos cell
Validation Images & Assay Conditions
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Western blot analysis of TCL1 expression in Ramos cell lysate.
Specific Publications For Anti-TCL1 Monoclonal Antibody (M03713)
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Customer Q&As
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5 Customer Q&As for Anti-TCL1 Monoclonal Antibody
Question
We are currently using anti-TCL1 Monoclonal antibody M03713 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The anti-TCL1 Monoclonal antibody (M03713) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-04
Question
Is there a BSA free version of anti-TCL1 Monoclonal antibody M03713 available?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-TCL1 Monoclonal antibody M03713 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-25
Question
Will anti-TCL1 Monoclonal antibody M03713 work on primate WB with oocyte?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Our lab technicians have not tested anti-TCL1 Monoclonal antibody M03713 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TCL1 Monoclonal antibody M03713 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-25
Question
Is this M03713 anti-TCL1 Monoclonal antibody reactive to the isotypes of TCL1A?
R. Patel
Verified customer
Asked: 2019-10-28
Answer
The immunogen of M03713 anti-TCL1 Monoclonal antibody is A synthesized peptide derived from human TCL1 Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-28
Question
Will M03713 anti-TCL1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
It shows on the product datasheet, M03713 anti-TCL1 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-29
