Product Info Summary
SKU: | M05524-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-TCP-1 beta CCT2 Monoclonal Antibody
SKU/Catalog Number
M05524-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TCP-1 beta CCT2 Monoclonal Antibody catalog # M05524-1. Tested in ELISA, Flow Cytometry, IF, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCP-1 beta CCT2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05524-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5B5C4
Isotype
IgG
Immunogen
Purified recombinant fragment of human TCP-1 beta expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M05524-1 is reactive to CCT2 in Human, Monkey, Mouse, Rat
Applications
M05524-1 is guaranteed for ELISA, Flow Cytometry, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
57.488kDa
Background of TCP1-beta
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. ELISA validation of CCT2 using Anti-TCP-1 beta CCT2 Monoclonal Antibody (M05524-1).
ELISA analysis of TCP-1 beta antibody.Click image to see more details
Figure 3. Flow Cytometry validation of CCT2 using Anti-TCP-1 beta CCT2 Monoclonal Antibody (M05524-1).
Flow cytometric (FCM) analysis of NIH/3T3 cells using TCP-1 beta Monoclonal Antibody (blue) and negative control (red).Click image to see more details
Figure 2. Immunofluorescent staining data of CCT2 using Anti-TCP-1 beta CCT2 Monoclonal Antibody (M05524-1).
Immunofluorescence (IF) analysis of 3T3-L1 cells using TCP-1 beta Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 1. Western blotting validation for Anti-TCP-1 beta CCT2 Monoclonal Antibody M05524-1
Western Blot (WB) analysis using TCP-1 beta Monoclonal Antibody against HeLa (1)Protein Target Info & Infographic
Gene/Protein Information For CCT2 (Source: Uniprot.org, NCBI)
Gene Name
CCT2
Full Name
T-complex protein 1 subunit beta
Weight
57.488kDa
Superfamily
TCP-1 chaperonin family
Alternative Names
Cctb; CCT-beta; CCTBPRO1633,99D8.1; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); MGC142074; MGC142076; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; TCP-1-beta CCT2 99D8.1, CCT-beta, CCTB, HEL-S-100n, PRO1633, TCP-1-beta chaperonin containing TCP1 subunit 2 T-complex protein 1 subunit beta|T-complex protein 1, beta subunit|chaperonin containing TCP1, subunit 2 (beta)|chaperonin containing t-complex polypeptide 1, beta subunit|chaperonin containing t-complex polypeptide 1, subunit 2|epididymis secretory sperm binding protein Li 100n
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCT2, check out the CCT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCP-1 beta CCT2 Monoclonal Antibody (M05524-1)
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No publications found for M05524-1
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