Anti-TCPTP/PTPN2 Antibody Picoband™

Product Name

Anti-TCPTP/PTPN2 Antibody Picoband™

View all TC-PTP/PTPN2 Antibodies

SKU/Catalog Number

PB9501

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TCPTP/PTPN2 Antibody Picoband™ catalog # PB9501. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TCPTP/PTPN2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9501)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TCPTP recombinant protein (Position: M1-C330). Human TCPTP shares 91.4% and 92% amino acid (aa) sequence identity with mouse and rat TCPTP, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9501 is reactive to PTPN2 in Human, Rat

Applications

PB9501 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

48.473kDa

Background of TC-PTP/PTPN2

TCPTP is also called as PTPN2. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TCPTP using anti-TCPTP antibody (PB9501).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug,
Lane 2: JURKAT Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCPTP antigen affinity purified polyclonal antibody (Catalog # PB9501) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCPTP at approximately 48 kDa. The expected band size for TCPTP is at 48 kDa.

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Figure 2. IHC analysis of TCPTP using anti-TCPTP antibody (PB9501).
TCPTP was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TCPTP Antibody (PB9501) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of TCPTP using anti-TCPTP antibody (PB9501).
TCPTP was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TCPTP Antibody (PB9501) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For PTPN2 (Source: Uniprot.org, NCBI)

Alternative Names

protein tyrosine phosphatase, non-receptor type 2; PTP2; PTPN2; PTPTPTN2; T-cell protein tyrosine phosphatase; T-cell protein-tyrosine phosphatase; TCELLPTP; TCPTP; TC-PTP; TCPTPEC 3.1.3.48; tyrosine-protein phosphatase non-receptor type 2 PTPN2 PTN2, PTPT, TC-PTP, TCELLPTP, TCPTP protein tyrosine phosphatase non-receptor type 2 tyrosine-protein phosphatase non-receptor type 2|T-cell protein tyrosine phosphatase

For more info on PTPN2, check out the PTPN2 Infographic

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In this infographic, you will see the following information for PTPN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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