Product Info Summary
| SKU: | A10047 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Teneurin-3 TENM3 Antibody
SKU/Catalog Number
A10047
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Teneurin-3 TENM3 Antibody (Catalog # A10047). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TENM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Teneurin-3 TENM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10047)
Host
Rabbit
Contents
TENM3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TENM3 antibody was raised against a 17 amino acid peptide near the amino terminus of human TENM3. The immunogen is located within the first 50 amino acids of TENM3.
Cross-reactivity
TENM3 antibody is human, mouse and rat reactive. TENM3 antibody is predicted to not cross-react with other members of the TENM family.
Reactive Species
A10047 is reactive to TENM3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
301.0 kDa
Background of TENM3
The teneurin transmembrane protein 3 (TENM3) is a member of a family of four neuronal cell surface proteins homologous to the Drosophila pair-rule gene Ten-m (1,2). TENM3 is expressed in cartilaginous cells during postnatal growth in mice (3), as well as the adult eye and optic stalk (4). Homozygous null mutations in TENM3 causes microphthalmia in humans (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10047 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TENM3 antibody can be used for detection of TENM3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
WB Validation in 293 Cells
Loading: 15 μg of Cell lysate
Antibodies: TENM3 A10047-8152-2402, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
WB Validation in Mouse and Rat Brain
Loading: 15 μg of Cell lysate
Antibodies: TENM3 A10047-8152-2402, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunohistochemistry of TENM3 in mouse brain tissue with TENM3 antibody at 5 μg/mL.
Click image to see more details
Immunofluorescence of TENM3 in mouse brain tissue with TENM3 antibody at 20 μg/mL.
Specific Publications For Anti-Teneurin-3 TENM3 Antibody (A10047)
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