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Product Info Summary
| SKU: | A01917 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-TH Antibody (C-term)
View all Tyrosine Hydroxylase Antibodies
SKU/Catalog Number
A01917
Size
80 µl
Description
Boster Bio Anti-TH Antibody (C-term) (Catalog # A01917). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TH Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01917)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of human TH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01917 is reactive to TH in Human
Reconstitution
Calculated molecular weight
58600 Da
Background of Tyrosine Hydroxylase
TH is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01917 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody (C-term). A01917 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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TH Antibody (C-term) (Cat. #A01917) western blot analysis in SH-SY5Y cell line lysates (35ug/lane). This demonstrates the TH antibody detected the TH protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue with TH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of K562 cells using TH Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For TH (Source: Uniprot.org, NCBI)
Gene Name
TH
Full Name
Tyrosine 3-monooxygenase
Weight
58600 Da
Superfamily
biopterin-dependent aromatic amino acid hydroxylase family
Alternative Names
Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, TH, TYH TH DYT14, DYT5b, TYH tyrosine hydroxylase tyrosine 3-monooxygenase|dystonia 14|tyrosine 3-hydroxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TH, check out the TH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TH Antibody (C-term) (A01917)
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