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Product Info Summary
| SKU: | A01917 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-TH Antibody (C-term)
SKU/Catalog Number
A01917
Size
80 µl
Description
Boster Bio Anti-TH Antibody (C-term) (Catalog # A01917). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TH Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01917)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of human TH.
Reactive Species
A01917 is reactive to TH in Human
Calculated molecular weight
58.6 kDa
Background of TH
TH is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01917 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody (C-term). A01917 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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TH Antibody (C-term) (Cat. #A01917) western blot analysis in SH-SY5Y cell line lysates (35ug/lane). This demonstrates the TH antibody detected the TH protein (arrow).
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Formalin-fixed and paraffin-embedded human brain tissue with TH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of K562 cells using TH Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-TH Antibody (C-term) (A01917)
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