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Product Info Summary
| SKU: | A14419 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-THAP4 Antibody
SKU/Catalog Number
A14419
Size
100μl
Form
Liquid
Description
Boster Bio Anti-THAP4 Antibody catalog # A14419. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-THAP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14419)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human THAP4. AA range:341-390
Reactive Species
A14419 is reactive to THAP4 in Human, Monkey, Mouse, Rat
Calculated molecular weight
62.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14419 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using THAP4 Polyclonal Antibody
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Western blot analysis of the lysates from HT-29 cells using THAP4 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-THAP4 Antibody (A14419)
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Customer Q&As
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6 Customer Q&As for Anti-THAP4 Antibody
Question
Is a blocking peptide available for product anti-THAP4 antibody (A14419)?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do provide the blocking peptide for product anti-THAP4 antibody (A14419). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-28
Question
Is there a BSA free version of anti-THAP4 antibody A14419 available?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-THAP4 antibody A14419 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-08
Question
Will A14419 anti-THAP4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Williams
Verified customer
Asked: 2019-11-19
Answer
You can see on the product datasheet, A14419 anti-THAP4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-19
Question
Will anti-THAP4 antibody A14419 work on horse WB with brain?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
Our lab technicians have not validated anti-THAP4 antibody A14419 on horse. You can run a BLAST between horse and the immunogen sequence of anti-THAP4 antibody A14419 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-24
Question
We are currently using anti-THAP4 antibody A14419 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
S. Singh
Verified customer
Asked: 2014-08-05
Answer
The anti-THAP4 antibody (A14419) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-08-05
Question
Can you help my question with product A14419, anti-THAP4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Zhao
Verified customer
Asked: 2013-09-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14419 anti-THAP4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-09-24
