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Product Info Summary
| SKU: | M01775 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Thyroid Hormone Receptor alpha (THRA) Mouse Monoclonal Antibody [Clone ID: OTI2A2]
SKU/Catalog Number
M01775
Size
100 µl
Description
Boster Bio THRA mouse monoclonal antibody, clone OTI2A2 (formerly 2A2). Catalog# M01775. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Thyroid Hormone Receptor alpha (THRA) Mouse Monoclonal Antibody [Clone ID: OTI2A2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01775)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2A2
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-220 of human THRA (NP_955366) produced in E.coli.
Reactive Species
M01775 is reactive to THRA in Human, Mouse, Rat
Calculated molecular weight
54.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01775 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY THRA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-THRA.
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-THRA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-THRA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
Specific Publications For Anti-Thyroid Hormone Receptor alpha (THRA) Mouse Monoclonal Antibody [Clone ID: OTI2A2] (M01775)
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