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Product Info Summary
| SKU: | A05811-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-TIGAR Antibody
View all TIGAR/C12orf5 Antibodies
SKU/Catalog Number
A05811-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TIGAR Antibody (Catalog # A05811-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TIGAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TIGAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05811-1)
Host
Rabbit
Contents
TIGAR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TIGAR antibody was raised against a 18 amino acid synthetic peptide from near the center of human TIGAR. The immunogen is located within amino acids 60 - 110 of TIGAR.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
MKRN3 antibody is human specific. MKRN3 antibody is predicted to not cross-react with other members of the MKRN protein family.
Reactive Species
A05811-1 is reactive to TIGAR in Human, Mouse, Rat
Applications
A05811-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
30.063kDa
Background of TIGAR/C12orf5
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death; loss of its functions contributes to the development of most cancers. Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase. TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress. TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TIGAR antibody can be used for detection of TIGAR by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TIGAR in EL4 cell lysate with TIGAR antibody at (A) 0.5
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Immunohistochemistry of TIGAR in human brain tissue with TIGAR antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TIGAR (Source: Uniprot.org, NCBI)
Gene Name
TIGAR
Full Name
Fructose-2,6-bisphosphatase TIGAR
Weight
30.063kDa
Superfamily
phosphoglycerate mutase family
Alternative Names
C12orf5; chromosome 12 open reading frame 5; EC 3.1.3.46; probable fructose-2,6-bisphosphatase TIGAR; TIGAR; TIGARTP53-induced glycolysis and apoptosis regulator; transactivated by NS3TP2 protein TIGAR C12orf5, FR2BP TP53 induced glycolysis regulatory phosphatase fructose-2,6-bisphosphatase TIGAR|TP53-induced glycolysis and apoptosis regulator|fructose-2,6-bisphosphate 2-phosphatase|probable fructose-2,6-bisphosphatase TIGAR|transactivated by NS3TP2 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TIGAR, check out the TIGAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TIGAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIGAR Antibody (A05811-1)
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No publications found for A05811-1
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