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- Table of Contents
TIMM23 antibody (A09218-1) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of TIMM23 using anti-TIMM23 antibody (A09218-1).
TIMM23 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-TIMM23 Antibody (A09218-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
TIMM23 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-TIMM23 Antibody (A09218-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-TIM23/TIMM23 Antibody Picoband®
TIMM23 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
TIMM23 antibody was positively detected by WB in:
human HepG2 whole cell, human Jurkat whole cell, human MCF-7 whole cell, human Caco-2 whole cell, rat heart tissue, mouse heart tissue, human A549 whole cell, human 293T whole cell, rat kidney tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TIMM23 antibody was positively detected by IHC in:
human colon cancer tissue, human thyroid cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-TIM23/TIMM23 Antibody Picoband®
View all TIMM23 Antibodies
View all TIMM23 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TIM23/TIMM23.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
20 kDa
Calculated Molecular Weight
21.9 kDa
Background of TIMM23
The protein encoded by this gene is part of a complex located in the inner mitochondrial membrane that mediates the transport of transit peptide-containing proteins across the membrane. Multiple transcript variants, one protein-coding and others not protein-coding, have been found for this gene.
Cite This Product
Anti-TIM23/TIMM23 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09218-1)
Protocols
Western Blot protocol for TIMM23 antibody
Immunohistochemistry (IHC) protocol for TIMM23 antibody
Publications
Application:
Species:
Subcellular Localization of Telomerase Reverse Transcriptase Regulates Gene-Specific Modulation of Mitochondrial DNA Copy Number in Neuronal Cells
Published: 2026-07-08
Journal: MOLECULAR NEUROBIOLOGY
Application: IF
Impact Factor: 5.50
Species: Mouse
PMID: 42420567
Neuroprotection and safety profile of Astragalus membranaceus extract TA-65 in neonatal hypoxic-ischemic brain damage by activating mitochondrial telomerase reverse transcriptase: Evidence from in vitro and in vivo studies
Published: 2025-05-31
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IF
Impact Factor: 5.40
Species: Mouse
PMID: 40456473
Review
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Product Promise
We promise Anti-TIM23/TIMM23 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A09218-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026