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F3 antibody (PB9701) performs well in Flow Cytometry, IF, IHC, ICC, WB, ELISA (Cap) and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of A431 cells using anti-Tissue Factor antibody (PB9701).
Overlay histogram showing A431 cells stained with PB9701 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tissue Factor Antibody (PB9701, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with PB9701 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tissue Factor Antibody (PB9701, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Tissue Factor/F3 Antibody Picoband®
F3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IF, IHC, ICC, WB, ELISA (Cap) |
Tested Applications
F3 antibody was positively detected by WB in:
human A431 whole cell, human Hacat whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
F3 antibody was positively detected by IHC in:
human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
F3 antibody was positively detected by IF in:
Figure 3 IF analysis of tissue
F3 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA(Cap)
1-5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Tissue Factor/F3 Antibody Picoband®
View all F3 Antibodies
View all F3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tissue Factor recombinant protein (Position: S33-S295). Human Tissue Factor shares 57.6% and 57.2% amino acid (aa) sequence identity with mouse and rat Tissue Factor, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
33.1 kDa
Background of F3
Tissue factor also called platelet tissue factor, factor III, or CD142. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
Cite This Product
Anti-Tissue Factor/F3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9701)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
94%
High Homology
Dog
72%
Moderate Homology
Rabbit
71%
Moderate Homology
Cow
70%
Moderate Homology
Sheep
70%
Moderate Homology
Rat
57%
Low homology
Mouse
57%
Low homology
Zebrafish
37%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for F3 antibody
Immunohistochemistry (IHC) protocol for F3 antibody
Immunofluorescence (IF) protocol for F3 antibody
Flow Cytometry (FC) protocol for F3 antibody
Publications
Application:
Guo Z, Qi W, Yu Y, Du S, Wu J, Liu J. Diabetol Metab Syndr. 2014 Feb 28;6(1):29. Doi: 10.1186/1758-5996-6-29. Effect Of Exenatide On The Cardiac Expression Of Adiponectin Receptor 1 And Nadph Oxidase Subunits And Heart Function In Streptozotocin-I...
PMID: 24576329
Guo Z, Zhang R, Li J, Xu G. Cardiovasc Diabetol. 2012 Aug 8;11:94. Doi: 10.1186/1475-2840-11-94. Effect Of Telmisartan On The Expression Of Adiponectin Receptors And Nicotinamide Adenine Dinucleotide Phosphate Oxidase In The Heart And Aorta In Typ...
PMID: 22873349
Li Y, Cao Y, Zeng Z, Liang M, Xue Y, Xi C, Zhou M, Jiang W. Sci Rep. 2015 Feb 3;5:8209. Doi: 10.1038/Srep08209. Angiotensin-Converting Enzyme 2/Angiotensin-(1-7)/Mas Axis Prevents Lipopolysaccharide-Induced Apoptosis Of Pulmonary Microvascular End...
PMID: 25644821
Zhu W, Lv Q, Chen H, Wang Z, Zhong Q. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):441-5. Doi: 10.1007/S11596-011-0470-8. Epub 2011 Aug 7. Protective Effect And Mechanism Of Sodium Tanshinone Ii A Sulfonate On Microcirculatory Disturbance...
PMID: 21823002
Li X, Li M, Li Y, Quan Q, Wang J. Neural Regen Res. 2012 Dec 25;7(36):2860-6. Doi: 10.3969/J.Issn.1673-5374.2012.36.002. Cellular And Molecular Mechanisms Underlying The Action Of Ginsenoside Rg1 Against Alzheimer'S Disease.
PMID: 25317137
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Product Promise
We promise Anti-Tissue Factor/F3 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
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PB9701
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026