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Product Info Summary
| SKU: | A05309-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TKR/TKTL1 Antibody Picoband®
SKU/Catalog Number
A05309-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TKR/TKTL1 Antibody Picoband® catalog # A05309-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TKR/TKTL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05309-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TKR/TKTL1, which shares 68.2% amino acid (aa) sequence identity with mouse TKTL1.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05309-1 is reactive to TKTL1 in Human, Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
65.3 kDa
Background of TKTL1
Transketolase-like protein 1 is an enzyme with a catalytic function that in humans is encoded by the TKTL1 gene. The protein encoded by this gene is a transketolase that acts as a homodimer and catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate. This reaction links the pentose phosphate pathway with the glycolytic pathway. Variations in this gene may be the cause of Wernicke-Korsakoff syndrome. Three transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05309-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MOLT-4 whole cell, rat testis tissue, rat PC-12 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TKR/TKTL1 using anti-TKR/TKTL1 antibody (A05309-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse testis tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TKR/TKTL1 antigen affinity purified polyclonal antibody (Catalog # A05309-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TKR/TKTL1 at approximately 65 kDa. The expected band size for TKR/TKTL1 is at 65 kDa.
Specific Publications For Anti-TKR/TKTL1 Antibody Picoband® (A05309-1)
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