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Product Info Summary
| SKU: | M00131-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-TLR2 Antibody
SKU/Catalog Number
M00131-1
Size
50 µl
Description
Boster Bio Anti-TLR2 Antibody (Catalog # M00131-1). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TLR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00131-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1070CT4.2.3.2
Isotype
IgG1,κ
Immunogen
This TLR2 Antibody is generated from a mouse immunized with a recombinant protein .
Reactive Species
M00131-1 is reactive to TLR2 in Human
Calculated molecular weight
89.8 kDa
Background of TLR2
Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00131-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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All lanes : Anti-TLR2 Antibody at 1:500-1:1000 dilution
Lane 1: Ramos whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: MDA-MB-231 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 85 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-TLR2 Antibody (M00131-1)
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