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Product Info Summary
| SKU: | M00198 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TLR9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00198
BM5150 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TLR9 Rabbit Monoclonal Antibody catalog # M00198. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00198)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABFH-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TLR9
Reactive Species
M00198 is reactive to TLR9 in Human
Observed Molecular Weight
130 kDa
Calculated molecular weight
115.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00198 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of TLR9 expression in Raji cell lysate.
Specific Publications For Anti-TLR9 Rabbit Monoclonal Antibody (M00198)
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5 Customer Q&As for Anti-TLR9 Rabbit Monoclonal Antibody
Question
Will anti-TLR9 Rabbit Monoclonal antibody M00198 work on pig WB with lymph?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not tested anti-TLR9 Rabbit Monoclonal antibody M00198 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TLR9 Rabbit Monoclonal antibody M00198 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig lymph in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-TLR9 Rabbit Monoclonal antibody M00198 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The anti-TLR9 Rabbit Monoclonal antibody (M00198) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-12
Question
Will anti-TLR9 Rabbit Monoclonal antibody M00198 work for WB with blood?
Verified Customer
Verified customer
Asked: 2018-09-27
Answer
According to the expression profile of blood, TLR9 is highly expressed in blood. So, it is likely that anti-TLR9 Rabbit Monoclonal antibody M00198 will work for WB with blood.
Boster Scientific Support
Answered: 2018-09-27
Question
My question regarding product M00198, anti-TLR9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00198 anti-TLR9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-11
Question
My question regards to test anti-TLR9 Rabbit Monoclonal antibody M00198 on human blood for research purposes, then I may be interested in using anti-TLR9 Rabbit Monoclonal antibody M00198 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Carter
Verified customer
Asked: 2014-08-01
Answer
The products we sell, including anti-TLR9 Rabbit Monoclonal antibody M00198, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-08-01
