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Product Info Summary
| SKU: | A13431 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TM9SF2 Antibody Picoband®
SKU/Catalog Number
A13431
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TM9SF2 Antibody Picoband® catalog # A13431. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TM9SF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13431)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TM9SF2 recombinant protein (Position: R66-S596).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13431 is reactive to TM9SF2 in Human, Rat
Observed Molecular Weight
76 kDa
Calculated molecular weight
75.8 kDa
Background of TM9SF2
Transmembrane 9 superfamily member 2 is a protein that in humans is encoded by the TM9SF2 gene. This gene encodes a member of the transmembrane 9 superfamily. The encoded 76 kDa protein localizes to early endosomes in human cells. The encoded protein possesses a conserved and highly hydrophobic C-terminal domain which contains nine transmembrane domains. The protein may play a role in small molecule transport or act as an ion channel. A pseudogene associated with this gene is located on the X chromosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13431 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human THP-1 whole cell, rat PC-12 whole cell
Validation Images & Assay Conditions
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Western blot analysis of TM9SF2 using anti-TM9SF2 antibody (A13431).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM9SF2 antigen affinity purified polyclonal antibody (Catalog # A13431) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM9SF2 at approximately 76 kDa. The expected band size for TM9SF2 is at 76 kDa.
Specific Publications For Anti-TM9SF2 Antibody Picoband® (A13431)
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