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Product Info Summary
| SKU: | A12647-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TMED4 Antibody Picoband®
SKU/Catalog Number
A12647-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMED4 Antibody Picoband® catalog # A12647-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TMED4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12647-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TMED4, identical to the related mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12647-1 is reactive to TMED4 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.9 kDa
Background of TMED4
Transmembrane emp24 domain-containing protein 4 is a protein that in humans is encoded by the TMED4 gene. Involved in positive regulation of I-kappaB kinase/NF-kappaB signaling. Predicted to be located in endoplasmic reticulum membrane. Predicted to be integral component of membrane. Predicted to be active in several cellular components, including COPII-coated ER to Golgi transport vesicle; Golgi apparatus; and endoplasmic reticulum-Golgi intermediate compartment.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12647-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
Positive Control
WB: human HepG2 whole cell, human T-47D whole cell, human Hela whole cell, human SH-SY5Y whole cell, rat liver tissue, rat stomach tissue, mouse liver tissue, mouse stomach tissue
Validation Images & Assay Conditions
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Western blot analysis of TMED4 using anti-TMED4 antibody (A12647-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat stomach tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse stomach tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED4 antigen affinity purified polyclonal antibody (Catalog # A12647-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMED4 at approximately 25 kDa. The expected band size for TMED4 is at 26 kDa.
Specific Publications For Anti-TMED4 Antibody Picoband® (A12647-1)
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