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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-Transmembrane protein 199 TMEM199 Antibody
Boster Bio Anti-Transmembrane protein 199 TMEM199 Antibody catalog # A14686-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transmembrane protein 199 TMEM199 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14686-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human TMEM199 recombinant protein (Position: E20-H129).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A14686-1 is reactive to TMEM199 in Human, Mouse, Rat
A14686-1 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TMEM199 using anti-TMEM199 antibody (A14686-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: human U-87MG whole cell lysates,
Lane 6: human K562 whole cell lysates,
Lane 7: human U2OS whole cell lysates,
Lane 8: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM199 antigen affinity purified polyclonal antibody (Catalog # A14686-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM199 at approximately 23KD. The expected band size for TMEM199 is at 23KD.
Gene/Protein Information For TMEM199 (Source: Uniprot.Org, NCBI)
Transmembrane protein 199
C17orf32; chromosome 17 open reading frame 32; MGC45714; transmembrane protein 199 TMEM199 C17orf32, CDG2P, VMA12, VPH2 transmembrane protein 199 transmembrane protein 199*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TMEM199, check out the TMEM199 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TMEM199: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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