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Product Info Summary
| SKU: | A08336-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TMEM97 Antibody Picoband®
SKU/Catalog Number
A08336-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMEM97 Antibody Picoband® catalog # A08336-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TMEM97 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08336-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TMEM97 recombinant protein (Position: A5-L141).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08336-1 is reactive to TMEM97 in Human, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
20.8 kDa
Background of TMEM97
TMEM97 is a conserved integral membrane protein that plays a role in controlling cellular cholesterol levels .
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08336-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human CACO-2 whole cell, human RT4 whole cell, human HepG2 whole cell, human Daudi whole cell, rat testis tissue, rat pancreas tissue
Validation Images & Assay Conditions
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Western blot analysis of TMEM97 using anti-TMEM97 antibody (A08336-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human CACO-2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM97 antigen affinity purified polyclonal antibody (Catalog # A08336-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM97 at approximately 23 kDa. The expected band size for TMEM97 is at 21 kDa.
Specific Publications For Anti-TMEM97 Antibody Picoband® (A08336-1)
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