Anti-TMS1 PYCARD Rabbit Monoclonal Antibody

ASC/TMS1 antibody

Boster Bio Anti-TMS1 PYCARD Rabbit Monoclonal Antibody catalog # M00362. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00362
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

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Product Name

Anti-TMS1 PYCARD Rabbit Monoclonal Antibody

View all ASC/TMS1 Antibodies

SKU/Catalog Number

M00362

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TMS1 PYCARD Rabbit Monoclonal Antibody catalog # M00362. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TMS1 PYCARD Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00362)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FFB-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TMS1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00362 is reactive to PYCARD in Human

Applications

M00362 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

21.627kDa

Background of ASC/TMS1

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 150

Validation Images & Assay Conditions

Gene/Protein Information For PYCARD (Source: Uniprot.org, NCBI)

Gene Name

PYCARD

Full Name

Apoptosis-associated speck-like protein containing a CARD

Weight

21.627kDa

Alternative Names

ASC; ASCMGC10332; CARD5; CARD5TMS; caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; PYCARD; PYD and CARD domain containing; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; TMS1; TMS-1; TMS1apoptosis-associated speck-like protein containing a CARD PYCARD ASC, CARD5, TMS, TMS-1, TMS1 PYD and CARD domain containing apoptosis-associated speck-like protein containing a CARD|caspase recruitment domain-containing protein 5|target of methylation-induced silencing 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PYCARD, check out the PYCARD Infographic

PYCARD infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PYCARD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-TMS1 PYCARD Rabbit Monoclonal Antibody

Question

We are currently using anti-TMS1 Rabbit Monoclonal antibody M00362 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

The anti-TMS1 Rabbit Monoclonal antibody (M00362) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-16

Order DetailsPrice
M00362

100μL

$370
M00362-30ul

30μL sample

$99
M00362-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M00362
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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