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TNFRSF1A antibody (A00294-3) performs well in IHC, WB, ELISA (Cap) and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of TNFRSF1A using anti-TNFRSF1A antibody (A00294-3).
TNFRSF1A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFRSF1A Antibody (A00294-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
TNFRSF1A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFRSF1A Antibody (A00294-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-TNF Receptor I/TNFRSF1A Antibody Picoband®
TNFRSF1A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB, ELISA (Cap) |
Tested Applications
TNFRSF1A antibody was positively detected by WB in:
human Hela whole cell, human A549 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TNFRSF1A antibody was positively detected by IHC in:
mouse colon tissue, mouse kidney tissue, human brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
ELISA (Cap)
1-5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband®
View all TNFRSF1A Antibodies
View all TNFRSF1A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TNF Receptor I recombinant protein (Position: I22-T211).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
50.5 kDa
Background of TNFRSF1A
Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), also known as TNFR1, is a protein that in humans is encoded by the TNFRSF1A gene. The protein encoded by this gene is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. The TNFR1 gene is mapped to 12pter-cen. It encodes a protein of 455 amino acids. And this receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation.
Cite This Product
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00294-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
79%
Moderate Homology
Rabbit
76%
Moderate Homology
Mouse
71%
Moderate Homology
Rat
69%
Low homology
Cow
68%
Low homology
Sheep
68%
Low homology
Zebrafish
25%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TNFRSF1A antibody
Immunohistochemistry (IHC) protocol for TNFRSF1A antibody
Publications
Application:
Species:
Design of magnetic nanoparticles for hepatocellular carcinoma treatment using the control mechanisms of the cell internal nucleus and external membrane
Published: 2015-04-09
Journal: Journal of Materials Chemistry B
Application: WB
Impact Factor: 4.73
Species: Mouse
PMID: 32262296
Death signal transduction induced by co-immobilized TNF-α plus IFN-γ and the development of polymeric anti-cancer drugs
Published: 2010-09-15
Journal: BIOMATERIALS
Application: WB
Impact Factor: 7.37
Species: Human
PMID: 20832854
Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells
Published: 2009-05-04
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IHC
Impact Factor: 0.98
Species: Human
PMID: 19564935
Effect of Huwentoxin-I on the Fas and TNF apoptosis pathway in the hippocampus of rat with global cerebral ischemia
Published: 2007-08-07
Journal: TOXICON
Application: IHC
Impact Factor: 2.51
Species: Rat
PMID: 17900647
Expression and localization of tumor necrosis factor receptor 1 protein in the chorionic villi in early normal and spontaneous abortion
Published: 2006-07-20
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology
Application: IHC
Impact Factor: 1.14
Species: Human
PMID: 16859822
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Product Promise
We promise Anti-TNF Receptor I/TNFRSF1A Antibody Picoband® performs excellently in relevant applications for IHC, WB, ELISA (Cap). If it doesn't perform as promised, we will refund the product. Learn more
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A00294-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026