Anti-TNFAIP3 Antibody

Product Name

Anti-TNFAIP3 Antibody

View all TNFAIP3 Antibodies

SKU/Catalog Number

A00224-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TNFAIP3 Antibody (Catalog # A00224-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TNFAIP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TNFAIP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00224-1)

Host

Rabbit

Contents

TNFAIP3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TNFAIP3 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human TNFAIP3. The immunogen is located within the last 50 amino acids of TNFAIP3.

Reactive Species

A00224-1 is reactive to TNFAIP3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

87.7 kDa

Background of TNFAIP3

TNFAIP3, also known as A20, is located in chromosome band 6q23, a region that is often deleted in B cell lymphomas. Recently, it was identified as a tumor suppressor gene in Hodgkin lymphoma and several subtypes of non-Hodgkin lymphomas. TNFAIP3 was initially identified as a zinc-finger protein that is rapidly and transiently induced by TNF-α, inhibiting NF-κB-dependent gene expression, and protecting cells from TNF-α-cytotoxicity. Overexpression of TNFAIP3 also inhibits the TLR2- and TLR4-mediated interleukin-8 synthesis in airway epithelial cells, suggesting that TNFAIP3 also acts as a negative regulator of TLR-mediated inflammatory responses, thereby protecting the host against harmful over-responses to pathogens. At least two isoforms of TNFAIP3 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00224-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TNFAIP3 antibody can be used for detection of TNFAIP3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TNFAIP3

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