Overview
| Product Name |
Anti-TNFRSF1A Antibody Picoband™
See more TNF RI/TNFRSF1A products |
| Catalog# |
A00294 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
| Description |
Polyclonal antibody for TNF RI/TNFRSF1A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. TNF RI/TNFRSF1A information: Molecular Weight: 50495 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Golgi apparatus membrane ; Single- pass type I membrane protein . Secreted . A secreted form is produced through proteolytic processing. |
| Cite This Product |
Anti-TNFRSF1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00294)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-TNFRSF1A Antibody Picoband™ may replace the following items: sc 31350|sc 1067|sc 31349|sc 52739|sc 8436|sc 7895|sc 1069|sc 31351|sc 1068|sc 12746|sc 374185|sc 1070|sc 374186. |
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human, Mouse, Rat |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived human TNFRSF1A recombinant protein (Position: F89-N145). Human TNFRSF1A shares 67.3% and 69.1% amino acid (aa) sequence identity with mouse and rat TNFRSF1A, respectively. |
| Cross Reactivity |
No cross reactivity with other proteins. |
Gene/Protein Basic Information For TNFRSF1A (Source: Uniprot.org, NCBI)
| Uniprot Id | P19438 |
|---|
| NCBI Gene Id | 7132 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | TNFRSF1A |
|---|
| Protein Name | Tumor necrosis factor receptor superfamily member 1A |
|---|
| Alternative Names | TNF RI/TNFRSF1A|CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor |
|---|
| Molecular Weight | 50495 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TNFRSF1A, check out the TNFRSF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for TNFRSF1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the TNFRSF1A infographic
Our Boster Quality Guarantee for Anti-TNFRSF1A Antibody Picoband™ covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of TNFRSF1A using anti- TNFRSF1A antibody (A00294).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat gaster tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TNFRSF1A antigen affinity purified polyclonal antibody (Catalog # A00294) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF1A at approximately 60KD. The expected band size for TNFRSF1A is at 50KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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