Anti-TNFSF4 Antibody

Product Name

Anti-TNFSF4 Antibody

View all TNFSF4 Antibodies

SKU/Catalog Number

A02554-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TNFSF4 Antibody (Catalog # A02554-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TNFSF4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-TNFSF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02554-2)

Host

Rabbit

Contents

TNFSF4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Rabbit polyclonal TNFSF4 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TNFSF4. The immunogen is located within the last 50 amino acids of TNFSF4.

Cross-reactivity

At least two isoforms of TNFSF4 are known to exist; this antibody will detect both isoforms.

Reactive Species

A02554-2 is reactive to TNFSF4 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

21.1 kDa

Background of TNFSF4

The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNγ. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02554-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TNFSF4 antibody can be used for detection of TNFSF4 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TNFSF4

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