Anti-TOCA-1 FNBP1L Antibody

Product Name

Anti-TOCA-1 FNBP1L Antibody

View all FNBP1L Antibodies

SKU/Catalog Number

A06125

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TOCA-1 FNBP1L Antibody (Catalog # A06125). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TOCA-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TOCA-1 FNBP1L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06125)

Host

Rabbit

Contents

TOCA-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TOCA-1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human TOCA-1. The immunogen is located within the last 50 amino acids of TOCA-1.

Reactive Species

A06125 is reactive to FNBP1L in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

70.1 kDa

Background of FNBP1L

Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06125 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FNBP1L

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