Anti-Toll-like receptor 2 TLR2 Antibody

Product Name

Anti-Toll-like receptor 2 TLR2 Antibody

View all TLR2 Antibodies

SKU/Catalog Number

A00131-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Toll-like receptor 2 TLR2 Antibody (Catalog # A00131-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

TLR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Toll-like receptor 2 TLR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00131-1)

Host

Rabbit

Contents

TLR2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TLR2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TLR2. The immunogen is located within the first 50 amino acids of TLR2.

Reactive Species

A00131-1 is reactive to TLR2 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

89.8 kDa

Background of TLR2

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer can recognize a variety of bacterial and mycoplasma lipoproteins respectively.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00131-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TLR2 antibody can be used for detection of TLR2 by Western blot at 1 μg/mL. Despite its predicted molecular weight, TLR2 runs at ~100 kDa in SDS-PAGE. This polyclonal antibody can also detect TLR2 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information TLR2

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis