Anti-DNA topoisomerase 3-beta-1 TOP3B Antibody

Product Name

Anti-DNA topoisomerase 3-beta-1 TOP3B Antibody

View all Topo III beta Antibodies

SKU/Catalog Number

A10446

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-DNA topoisomerase 3-beta-1 TOP3B Antibody (Catalog # A10446). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DNA topoisomerase 3-beta-1 TOP3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10446)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human TOP3B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10446 is reactive to TOP3B in Human, Mouse

Applications

A10446 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

96.662kDa

Background of Topo III beta

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Possesses negatively supercoiled DNA relaxing activity.

Ng S.-W., Nucleic Acids Res. 27:993-1000(1999).
Kawasaki K., Genome Res. 7:250-261(1997).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from HepG2 cells, COLO205 cells and HUVEC cells, using TOP3B antibody A10446.

Gene/Protein Information For TOP3B (Source: Uniprot.org, NCBI)

Alternative Names

DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1; EC 5.99.1.2; FLJ39376; TOP3B1; topoisomerase (DNA) III beta; topoisomerase III beta TOP3B TOP3B1 DNA topoisomerase III beta DNA topoisomerase 3-beta-1|DNA topoisomerase III beta-1|topoisomerase (DNA) III beta|topoisomerase III beta

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