Product Info Summary
SKU: | A10446-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Topo II beta-1 TOP3B Antibody
View all Topo III beta Antibodies
SKU/Catalog Number
A10446-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Topo II beta-1 TOP3B Antibody catalog # A10446-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Topo II beta-1 TOP3B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10446-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Topo IIIbeta-1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10446-1 is reactive to TOP3B in Human, Mouse
Applications
A10446-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
96.662kDa
Background of Topo III beta
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Possesses negatively supercoiled DNA relaxing activity.
Ng S.-W., Nucleic Acids Res. 27:993-1000(1999).
Kawasaki K., Genome Res. 7:250-261(1997).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Topo II beta-1 TOP3B Antibody A10446-1
Western Blot (WB) analysis of specific cells using Topo IIIbeta-1 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For TOP3B (Source: Uniprot.org, NCBI)
Gene Name
TOP3B
Full Name
DNA topoisomerase 3-beta-1
Weight
96.662kDa
Superfamily
type IA topoisomerase family
Alternative Names
DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1; EC 5.99.1.2; FLJ39376; TOP3B1; topoisomerase (DNA) III beta; topoisomerase III beta TOP3B TOP3B1 DNA topoisomerase III beta DNA topoisomerase 3-beta-1|DNA topoisomerase III beta-1|topoisomerase (DNA) III beta|topoisomerase III beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOP3B, check out the TOP3B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOP3B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Topo II beta-1 TOP3B Antibody (A10446-1)
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No publications found for A10446-1
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