Anti-Topo III alpha TOP3A Antibody

TOP3A antibody

Boster Bio Anti-Topo III alpha TOP3A Antibody catalog # A07428. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A07428
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, WB

Customers Who Bought This Also Bought

Product Name

Anti-Topo III alpha TOP3A Antibody

View all TOP3A Antibodies

SKU/Catalog Number

A07428

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Topo III alpha TOP3A Antibody catalog # A07428. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Topo III alpha TOP3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07428)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

1A3

Isotype

IgG

Immunogen

Synthesized peptide derived from human Topo IIIalpha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07428 is reactive to TOP3A in Human, Mouse

Applications

A07428 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

112.372kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
ELISA 1:10000

Validation Images & Assay Conditions

Gene/Protein Information For TOP3A (Source: Uniprot.org, NCBI)

Gene Name

TOP3A

Full Name

DNA topoisomerase 3-alpha

Weight

112.372kDa

Superfamily

type IA topoisomerase family

Alternative Names

DNA topoisomerase 3-alpha TOP3A MGRISCE2, PEOB5, TOP3, ZGRF7 DNA topoisomerase III alpha DNA topoisomerase 3-alpha|topo III-alpha|topoisomerase (DNA) III alpha|zinc finger, GRF-type containing 7

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TOP3A, check out the TOP3A Infographic

TOP3A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TOP3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A07428

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Topo III alpha TOP3A Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

Be the first to review Anti-Topo III alpha TOP3A Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Anti-Topo III alpha TOP3A Antibody

Question

I would like to test anti-Topo III alpha antibody A07428 on human thalamus trachea for research purposes, then I may be interested in using anti-Topo III alpha antibody A07428 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

The products we sell, including anti-Topo III alpha antibody A07428, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-06-20

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thalamus trachea using anti-Topo III alpha antibody A07428. Let me know if you need anything else.

E. Mangal

Verified customer

Asked: 2017-09-13

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-13

Question

We are currently using anti-Topo III alpha antibody A07428 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

The anti-Topo III alpha antibody (A07428) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-28

Question

Will A07428 anti-Topo III alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

B. Parker

Verified customer

Asked: 2016-08-09

Answer

It shows on the product datasheet, A07428 anti-Topo III alpha antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-08-09

Question

I was wanting to use your anti-Topo III alpha antibody for WB for human thalamus trachea on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thalamus trachea identification?

N. Lewis

Verified customer

Asked: 2015-09-04

Answer

As indicated on the product datasheet, A07428 anti-Topo III alpha antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human thalamus trachea in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-09-04

Question

Here is the WB image, lot number and protocol we used for thalamus trachea using anti-Topo III alpha antibody A07428. Please let me know if you require anything else.

E. Li

Verified customer

Asked: 2013-10-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-22

Question

My question regarding product A07428, anti-Topo III alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

O. Bhatt

Verified customer

Asked: 2013-09-10

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07428 anti-Topo III alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-09-10

Order DetailsPrice
A07428

100uL

$399

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A07428
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.