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Product Info Summary
| SKU: | A11024 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TPRBK/TTC28 Antibody Picoband®
SKU/Catalog Number
A11024
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TPRBK/TTC28 Antibody Picoband® catalog # A11024. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TPRBK/TTC28 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11024)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TPRBK/TTC28 recombinant protein (Position: Q1586-D1950).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11024 is reactive to TTC28 in Human
Observed Molecular Weight
271 kDa
Calculated molecular weight
270.9 kDa
Background of TTC28
Tetratricopeptide repeat domain 28 is a protein that in humans is encoded by the TTC28 gene. Enables kinase binding activity. Involved in regulation of mitotic cell cycle. Located in midbody.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11024 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 0.1-0.25 μg/ml | Human |
| Flow Cytometry (FC) | 1-3 μg/1x106 cells | Human |
| ELISA | 0.1-0.5 μg/ml | - |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of TPRBK/TTC28 using anti-TPRBK/TTC28 antibody (A11024).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPRBK/TTC28 antigen affinity purified polyclonal antibody (Catalog # A11024) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPRBK/TTC28 at approximately 271 kDa. The expected band size for TPRBK/TTC28 is at 271 kDa.
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Flow Cytometry analysis of U87 cells using anti-TPRBK/TTC28 antibody (A11024).
Overlay histogram showing U87 cells stained with A11024 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPRBK/TTC28 Antibody (A11024, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-TPRBK/TTC28 Antibody Picoband® (A11024)
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