Anti-Transcription activator BRG1 SMARCA4 Antibody

Product Name

Anti-Transcription activator BRG1 SMARCA4 Antibody

View all Brg1 Antibodies

SKU/Catalog Number

A00223

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Transcription activator BRG1 SMARCA4 Antibody (Catalog # A00223). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SMARCA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Transcription activator BRG1 SMARCA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00223)

Host

Rabbit

Contents

SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4. The immunogen is located within amino acids 1420 - 1470 of SMARCA4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.

Reactive Species

A00223 is reactive to SMARCA4 in Human, Mouse, Rat

Applications

A00223 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

184.646kDa

Background of Brg1

The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1). Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2). The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3). In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.

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Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.

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Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.

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Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 μg/ml.

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Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.

Green: SMARCA4 Antibody (A00223)
Blue: DAPI staining

Gene/Protein Information For SMARCA4 (Source: Uniprot.org, NCBI)

Alternative Names

ATP-dependent helicase SMARCA4; BAF190; BAF190A; brahma protein-like 1; Brg1; BRG1-associated factor 190A; BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4; BRM/SWI2-related gene 1; EC 3.6.1; EC 3.6.4.-; FLJ39786; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; RTPS2; SMARCA4; SNF2; SNF2B; SNF2-beta; SNF2L4; SNF2LB; SNF2-like 4; subfamily a, member 4; sucrose nonfermenting-like 4; SWI/SNF related, matrix SMARCA4 BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 transcription activator BRG1|ATP-dependent helicase SMARCA4|BRG1-associated factor 190A|BRM/SWI2-related gene 1|SNF2-like 4|brahma protein-like 1|global transcription activator homologous sequence|homeotic gene regulator|mitotic growth and transcription activator|nuclear protein GRB1|protein BRG-1|protein brahma homolog 1|sucrose nonfermenting-like 4

For more info on SMARCA4, check out the SMARCA4 Infographic

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In this infographic, you will see the following information for SMARCA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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