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Product Info Summary
| SKU: | A00662 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Transcription factor EB TFEB Antibody
SKU/Catalog Number
A00662
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transcription factor EB TFEB Antibody (Catalog # A00662). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
TFEB antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Transcription factor EB TFEB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00662)
Host
Rabbit
Contents
TFEB Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TFEB antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TFEB. The immunogen is located within amino acids 70 - 120 of TFEB.
Reactive Species
A00662 is reactive to TFEB in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.9 kDa
Background of TFEB
The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00662 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TFEB antibody can be used for detection of TFEB by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TFEB in A549 cell lysate with TFEB antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB (NT) antibody at 2.5 μg/mL.
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Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 μg/mL.
Specific Publications For Anti-Transcription factor EB TFEB Antibody (A00662)
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