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- Table of Contents
SOX9 antibody (A00177-2) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 17 Publications
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IHC analysis of SOX9 using anti-SOX9 antibody (A00177-2).
SOX9 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
SOX9 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOX9 Antibody (A00177-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-SOX9 Antibody Picoband®
SOX9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
SOX9 antibody was positively detected by WB in:
human HepG2 whole cell, human PC-3 whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SOX9 antibody was positively detected by IHC in:
mouse lung tissue, mouse testis tissue, rat lung tissue, rat testis tissue, human intestinal cancer tissue, human intestinal cancer tissue, human intestinal cancer tissue, human mammary cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Tested
Recommend
Rat
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SOX9 Antibody Picoband®
View all SOX9 Antibodies
View all SOX9 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SOX9, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
56.1 kDa
Background of SOX9
Transcription factor SOX-9 is a protein that in humans is encoded by the SOX9 gene. The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Cite This Product
Anti-SOX9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00177-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Sheep
97%
High Homology
Zebrafish
86%
Moderate Homology
Drosophila
44%
Low homology
Cow
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SOX9 antibody
Immunohistochemistry (IHC) protocol for SOX9 antibody
Publications
Application:
Species:
Vanillic acid protects vertebral endplate chondrocytes against oxidative stress and inflammation via Nrf-2/HO-1 pathway
Published: 2026-07-23
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 5.70
Species: Mouse
PMID: 42492879
mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via Piezo1-NCOA4-ZBP1 axis
Published: 2026-05-20
Journal: Autophagy
Application: WB
Impact Factor: 18.60
Species: Mouse
PMID: 42153663
Ferristatin II protects nucleus pulposus against degeneration through inhibiting ferroptosis and activating HIF-1α pathway mediated mitophagy
Published: 2025-01-02
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Human
PMID: 39752759
Hoxc10‐mediated ‘positional memory’ regulates cartilage formation subsequent to femoral heterotopic grafting
Published: 2024-10-21
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: IF
Impact Factor: 4.30
Species: Rat
PMID: 39434203
HIF-2α/TFR1 mediated iron homeostasis disruption aggravates cartilage endplate degeneration through ferroptotic damage and mtDNA release: A new mechanism of intervertebral disc degeneration
Published: 2024-05-21
Journal: Journal of Orthopaedic Translation
Application: WB
Impact Factor: 5.90
Species: Mouse
PMID: 38808263
Remodeling of the pro-inflammatory microenvironment in osteoarthritis via hydrogel-based photothermal therapy
Published: 2024-02-20
Journal: Advanced Composites and Hybrid Materials
Application: WB
Impact Factor: 23.20
Species: Mouse
Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4‑mediated ferritinophagy
Published: 2023-12-07
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 38063237
Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy
Published: 2023-08-21
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: WB
Impact Factor: 10.20
Species: Mouse
PMID: 37605203
Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments
Published: 2023-06-01
Journal: BIOMEDICINE & PHARMACOTHERAPY
Application: WB
Impact Factor: 7.50
Species: Mouse,Human
PMID: 37269815
Novel strategy of senescence elimination via toxicity-exempted kinome perturbations by nanoliposome-based thermosensitive hydrogel for osteoarthritis therapy
Published: 2023-05-18
Journal: Advanced Composites and Hybrid Materials
Application: WB
Impact Factor: 20.10
Species: Mouse
Astaxanthin suppresses oxidative stress and calcification in vertebral cartilage endplate via activating Nrf-2/HO-1 signaling pathway
Published: 2023-04-11
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Mouse,Human
PMID: 37054647
Icariin protects vertebral endplate chondrocytes against apoptosis and degeneration via activating Nrf-2/HO-1 pathway
Published: 2022-09-13
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.99
Species: Mouse
PMID: 36176424
Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion
Published: 2019-10-17
Journal: Stem Cell Research & Therapy
Application: WB
Impact Factor: 4.63
Species: Mouse
PMID: 31623678
Glycosylation of dentin matrix protein 1 is critical for fracture healing via promoting chondrogenesis
Published: 2019-05-08
Journal: Frontiers of Medicine
Application: IHC
Impact Factor: 1.85
Species: Mouse
PMID: 31065929
Andrographolide prevents human nucleus pulposus cells against degeneration by inhibiting the NF-κB pathway
Published: 2018-10-28
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 3.92
Species: Human
PMID: 30370694
Intra-hydrogel culture prevents transformation of mesenchymal stem cells induced by monolayer expansion
Published: 2018-03-06
Journal: Biomaterials Science
Application: WB
Impact Factor: 5.83
Species: Human
PMID: 29564424
Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells
Published: 2016-10-18
Journal: Oncotarget
Application: WB
Impact Factor: 5.01
Species: Goat
PMID: 27769051
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Product Promise
We promise Anti-SOX9 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A00177-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026