Anti-TRIB3 Monoclonal Antibody

TRIB3 antibody

Boster Bio Anti-TRIB3 Monoclonal Antibody catalog # M01414. Tested in WB, IP applications. This antibody reacts with Human.

Product Info Summary

SKU: M01414
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, WB

Product Name

Anti-TRIB3 Monoclonal Antibody

View all TRIB3 Antibodies

SKU/Catalog Number

M01414

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TRIB3 Monoclonal Antibody catalog # M01414. Tested in WB, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRIB3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01414)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEIE-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TRIB3 Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01414 is reactive to TRIB3 in Human

Applications

M01414 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

39.578kDa

Background of TRIB3

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IP 1:20

Validation Images & Assay Conditions

Gene/Protein Information For TRIB3 (Source: Uniprot.org, NCBI)

Gene Name

TRIB3

Full Name

Tribbles homolog 3

Weight

39.578kDa

Superfamily

protein kinase superfamily

Alternative Names

C20orf97; dJ1103G7.3; neuronal cell death inducible putative kinase; Neuronal cell death-inducible putative kinase; NIPK; p65-interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; SINK; SKIP3; TRB-3; TRB3chromosome 20 open reading frame 97; tribbles homolog 3 (Drosophila); tribbles homolog 3 TRIB3 C20orf97, NIPK, SINK, SKIP3, TRB3 tribbles pseudokinase 3 tribbles homolog 3|TRB-3|neuronal cell death inducible putative kinase|p65-interacting inhibitor of NF-kappa-B|p65-interacting inhibitor of NF-kappaB

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TRIB3, check out the TRIB3 Infographic

TRIB3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TRIB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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8 Customer Q&As for Anti-TRIB3 Monoclonal Antibody

Question

Will anti-TRIB3 Monoclonal antibody M01414 work on horse IP with liver?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

Our lab technicians have not tested anti-TRIB3 Monoclonal antibody M01414 on horse. You can run a BLAST between horse and the immunogen sequence of anti-TRIB3 Monoclonal antibody M01414 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-03

Question

What is the sample target on Western blot analysis in your product image for M01414?

Verified customer

Asked: 2019-12-09

Answer

Western blot analysis of the Anti-TRIB3 Monoclonal Antibody (M01414) expression in 293T cell lysate.

Boster Scientific Support

Answered: 2019-12-10

Question

Is this M01414 anti-TRIB3 Monoclonal antibody reactive to the isotypes of TRIB3?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

The immunogen of M01414 anti-TRIB3 Monoclonal antibody is A synthesized peptide derived from human TRIB3 Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-03

Question

We are currently using anti-TRIB3 Monoclonal antibody M01414 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

The anti-TRIB3 Monoclonal antibody (M01414) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-05

Question

Is a blocking peptide available for product anti-TRIB3 Monoclonal antibody (M01414)?

Verified Customer

Verified customer

Asked: 2019-02-20

Answer

We do provide the blocking peptide for product anti-TRIB3 Monoclonal antibody (M01414). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-02-20

Question

Please see the WB image, lot number and protocol we used for liver using anti-TRIB3 Monoclonal antibody M01414. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-12-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-12-26

Question

Would M01414 anti-TRIB3 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

A. Krishna

Verified customer

Asked: 2018-08-16

Answer

You can see on the product datasheet, M01414 anti-TRIB3 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-16

Question

I see that the anti-TRIB3 Monoclonal antibody M01414 works with WB, what is the protocol used to produce the result images on the product page?

C. Krishna

Verified customer

Asked: 2017-11-06

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-11-06

Order DetailsPrice
M01414

100μL

$370
M01414-30ul

30μL sample

$99
M01414-carrier-free

Carrier Free

$370

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