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Product Info Summary
| SKU: | M01414 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-TRIB3 Monoclonal Antibody
SKU/Catalog Number
M01414
BM5628 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TRIB3 Monoclonal Antibody catalog # M01414. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRIB3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01414)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEIE-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TRIB3 Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity.
Reactive Species
M01414 is reactive to TRIB3 in Human
Observed Molecular Weight
40 kDa
Calculated molecular weight
39.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01414 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
Positive Control
WB: human K562 whole cell, human CACO-2 whole cell, human 293T whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TRIB3 using anti-TRIB3 antibody (M01414).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human CACO-2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIB3 antigen affinity purified monoclonal antibody (Catalog # M01414) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIB3 at approximately 40 kDa. The expected band size for TRIB3 is at 40 kDa.
Specific Publications For Anti-TRIB3 Monoclonal Antibody (M01414)
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Customer Q&As
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8 Customer Q&As for Anti-TRIB3 Monoclonal Antibody
Question
Will anti-TRIB3 Monoclonal antibody M01414 work on horse IP with liver?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Our lab technicians have not tested anti-TRIB3 Monoclonal antibody M01414 on horse. You can run a BLAST between horse and the immunogen sequence of anti-TRIB3 Monoclonal antibody M01414 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse liver in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-03
Question
What is the sample target on Western blot analysis in your product image for M01414?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-TRIB3 Monoclonal Antibody (M01414) expression in 293T cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
Is this M01414 anti-TRIB3 Monoclonal antibody reactive to the isotypes of TRIB3?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The immunogen of M01414 anti-TRIB3 Monoclonal antibody is A synthesized peptide derived from human TRIB3 Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-03
Question
We are currently using anti-TRIB3 Monoclonal antibody M01414 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
The anti-TRIB3 Monoclonal antibody (M01414) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-05
Question
Is a blocking peptide available for product anti-TRIB3 Monoclonal antibody (M01414)?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
We do provide the blocking peptide for product anti-TRIB3 Monoclonal antibody (M01414). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-02-20
Question
Please see the WB image, lot number and protocol we used for liver using anti-TRIB3 Monoclonal antibody M01414. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-26
Question
Would M01414 anti-TRIB3 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Krishna
Verified customer
Asked: 2018-08-16
Answer
You can see on the product datasheet, M01414 anti-TRIB3 Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-16
Question
I see that the anti-TRIB3 Monoclonal antibody M01414 works with WB, what is the protocol used to produce the result images on the product page?
C. Krishna
Verified customer
Asked: 2017-11-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-11-06
