Anti-TRIF/TICAM1 Antibody

Product Name

Anti-TRIF/TICAM1 Antibody

View all TRIF/TICAM1 Antibodies

SKU/Catalog Number

PA1902

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRIF/TICAM1 Antibody catalog # PA1902. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRIF/TICAM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1902)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TRIF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1902 is reactive to Ticam1 in Human

Applications

PA1902 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

79.23kDa

Background of TRIF/TICAM1

TICAM (TIR DOMAIN-CONTAINING ADAPTOR MOLECULE 1), also known as TICAM1 or TRIF, is an adapter in responding to activation of toll-like receptors (TLRs). It mediates the rather delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al. (2003) mapped the TICAM1 gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al. (2003) showed that TICAM1 interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TICAM1 mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TICAM1 activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. Small interfering (si)RNA blockage of TICAM1, just upstream of the TIR domain, reduced IFNB production in response to dsRNA.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-TRIF antibody, PA1902, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TICAM1, 44.7KD(162aa tag+ Q468-E712)
Lane 1: Recombinant Human TICAM1 Protein 10ng
Lane 2: Recombinant Human TICAM1 Protein 5ng
Lane 3: Recombinant Human TICAM1 Protein 2.5ng
Lane 4: Recombinant Human TICAM1 Protein 1.25ng

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Anti-TRIF antibody, PA1902, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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Figure 2. Western blot analysis of TRIF using anti-TRIF antibody (PA1902).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIF antigen affinity purified polyclonal antibody (Catalog # PA1902) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIF at approximately 110KD. The expected band size for TRIF is at 76KD.

Gene/Protein Information For Ticam1 (Source: Uniprot.org, NCBI)

Alternative Names

MGC35334; Proline-rich, vinculin and TIR domain-containing protein B; PRVTIRBTIR domain-containing adapter protein inducing IFN-beta; Putative NF-kappa-B-activating protein 502H; TICAM1; TICAM-1TIR domain containing adaptor inducing interferon-beta; TIR domain-containing adapter molecule 1; TIR domain-containing adaptor molecule 1; toll-like receptor adaptor molecule 1; TRIF; TRIFToll-interleukin-1 receptor domain-containing adapter protein inducinginterferon beta Ticam1|AW046014, AW547018, TICAM, TICAM-1, TRIF, Tr|toll-like receptor adaptor molecule 1|TIR domain-containing adapter molecule 1|TIR domain-containing adapter protein inducing IFN-beta|TIR domain-containing adaptor inducing interferon-beta|toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta

For more info on Ticam1, check out the Ticam1 Infographic

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In this infographic, you will see the following information for Ticam1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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