Anti-TRIM3 Antibody Picoband™

Product Name

Anti-TRIM3 Antibody Picoband™

View all TRIM3 Antibodies

SKU/Catalog Number

A07081-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TRIM3 Antibody Picoband™ catalog # A07081-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TRIM3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07081-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TRIM3 recombinant protein (Position: Q77-R433).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07081-2 is reactive to TRIM3 in Human, Mouse, Rat

Applications

A07081-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

81 kDa

Calculated molecular weight

80.83kDa

Background of TRIM3

Tripartite motif-containing protein 3 is a protein that in humans is encoded by the TRIM3 gene. The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TRIM3 using anti-TRIM3 antibody (A07081-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human T-47D whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM3 antigen affinity purified polyclonal antibody (Catalog # A07081-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM3 at approximately 81 kDa. The expected band size for TRIM3 is at 81 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-TRIM3 antibody (A07081-2).
Overlay histogram showing 293T cells stained with A07081-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM3 Antibody (A07081-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For TRIM3 (Source: Uniprot.org, NCBI)

Alternative Names

Tripartite motif-containing protein 3 TRIM3 BERP, HAC1, RNF22, RNF97 tripartite motif containing 3 tripartite motif-containing protein 3|RING finger protein 97|brain expressed ring finger|brain-expressed RING finger protein|ring finger protein 22|tripartite motif protein TRIM3

For more info on TRIM3, check out the TRIM3 Infographic

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In this infographic, you will see the following information for TRIM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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