Click image to see more details
Product Info Summary
| SKU: | A03133 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TRIM33 Antibody
SKU/Catalog Number
A03133
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM33 Antibody (Catalog # A03133). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TRIM33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TRIM33 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03133)
Host
Rabbit
Contents
TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33. The immunogen is located within amino acids 580 - 630 of TRIM33.
Cross-reactivity
TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.
Reactive Species
A03133 is reactive to TRIM33 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
122.5 kDa
Background of TRIM33
TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03133 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.
Click image to see more details
Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.
Specific Publications For Anti-TRIM33 Antibody (A03133)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TRIM33 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-TRIM33 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
