Product Info Summary
SKU: | A03133 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TRIM33 Antibody
View all TIF1 gamma Antibodies
SKU/Catalog Number
A03133
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM33 Antibody (Catalog # A03133). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TRIM33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TRIM33 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03133)
Host
Rabbit
Contents
TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33. The immunogen is located within amino acids 580 - 630 of TRIM33.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.
Reactive Species
A03133 is reactive to TRIM33 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
122533 MW
Background of TIF1 gamma
TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03133 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.
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Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For TRIM33 (Source: Uniprot.org, NCBI)
Gene Name
TRIM33
Full Name
E3 ubiquitin-protein ligase TRIM33
Weight
122533 MW
Superfamily
TRIM/RBCC family
Alternative Names
ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7 TRIM33 ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA tripartite motif containing 33 E3 ubiquitin-protein ligase TRIM33|RET-fused gene 7 protein|RING-type E3 ubiquitin transferase TRIM33|TIF1-gamma|ectodermin homolog|protein Rfg7|transcriptional intermediary factor 1 gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIM33, check out the TRIM33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIM33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRIM33 Antibody (A03133)
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