Product Info Summary
| SKU: | PB9272 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TRP 7/TRPC7 Antibody Picoband®
SKU/Catalog Number
PB9272
PB0272 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRP 7/TRPC7 Antibody Picoband® catalog # PB9272. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRP 7/TRPC7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9272)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRP 7 recombinant protein (Position: K716-I862). Human TRP 7 shares 95% amino acid (aa) sequence identity with mouse TRP 7.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9272 is reactive to TRPC7 in Human, Mouse
Observed Molecular Weight
99 kDa
Calculated molecular weight
99.6 kDa
Background of TRPC7
Transient receptor potential cation channel, subfamily C, member 7, also known as TRPC7, is a human gene encoding a protein of the same name. Using a cosmid/BAC contig, this gene is mapped to chromosome 21q22.3. TRPC7 is the most abundantly expressed long TRPC in peripheral blood and in some blood cell lines. The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9272 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: Mouse Brain Tissue, A549 Whole Cell, COLO320 Whole Cell, SKOV Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-TRP 7 Picoband antibody, PB9272, Western blotting
All lanes: Anti TRP 7 (PB9272) at 0.5ug/ml
WB: Recombinant Human TRP 7 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Click image to see more details
Anti-TRP 7 Picoband antibody, PB9272, Western blotting
All lanes: Anti TRP 7 (PB9272) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
Specific Publications For Anti-TRP 7/TRPC7 Antibody Picoband® (PB9272)
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Customer Q&As
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5 Customer Q&As for Anti-TRP 7/TRPC7 Antibody Picoband®
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-TRP 7/TRPC7 antibody PB9272. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-06
Question
I see that the anti-TRP 7/TRPC7 antibody PB9272 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-10-16
Question
I am interested in to test anti-TRP 7/TRPC7 antibody PB9272 on mouse buccal mucosa cell for research purposes, then I may be interested in using anti-TRP 7/TRPC7 antibody PB9272 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
The products we sell, including anti-TRP 7/TRPC7 antibody PB9272, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-02
Question
We are currently using anti-TRP 7/TRPC7 antibody PB9272 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on goat tissues as well?
L. Jackson
Verified customer
Asked: 2017-06-27
Answer
The anti-TRP 7/TRPC7 antibody (PB9272) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-27
Question
Do you have a BSA free version of anti-TRP 7/TRPC7 antibody PB9272 available?
O. Anderson
Verified customer
Asked: 2016-06-03
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRP 7/TRPC7 antibody PB9272 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-06-03


