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Product Info Summary
| SKU: | PB9903 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TRPM5 Antibody Picoband®
SKU/Catalog Number
PB9903
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TRPM5 Antibody Picoband® catalog # PB9903. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TRPM5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9903)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPM5 recombinant protein (Position: S1021-Q1108). Human TRPM5 shares 87.5% amino acid (aa) sequence identity with mouse TRPM5.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9903 is reactive to TRPM5 in Human, Mouse, Rat
Observed Molecular Weight
131 kDa
Calculated molecular weight
131.5 kDa
Background of TRPM5
Transient receptor potential cation channel subfamily M member 5 (TRPM5) is a protein that in humans is encoded by the TRPM5 gene. This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca (2+) ions. It is activated by lower concentrations of intracellular Ca (2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9903 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC whole cell, 293T whole cell
IHC: mouse brain tissue, rat testis tissue, human prostatic cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SMMC whole cell lysates,
Lane 2: 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM5 antigen affinity purified polyclonal antibody (Catalog # PB9903) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM5 at approximately 131 kDa. The expected band size for TRPM5 is at 131 kDa.
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IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
TRPM5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
TRPM5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903).
TRPM5 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-TRPM5 Antibody Picoband® (PB9903)
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7 Customer Q&As for Anti-TRPM5 Antibody Picoband®
Question
We are currently using anti-TRPM5 antibody PB9903 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The anti-TRPM5 antibody (PB9903) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-04
Question
I was wanting to use your anti-TRPM5 antibody for WB for mouse mucosa of transverse colon on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse mucosa of transverse colon identification?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
As indicated on the product datasheet, PB9903 anti-TRPM5 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse mucosa of transverse colon in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-17
Question
Will anti-TRPM5 antibody PB9903 work for WB with mucosa of transverse colon?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
According to the expression profile of mucosa of transverse colon, TRPM5 is highly expressed in mucosa of transverse colon. So, it is likely that anti-TRPM5 antibody PB9903 will work for WB with mucosa of transverse colon.
Boster Scientific Support
Answered: 2020-03-03
Question
Would PB9903 anti-TRPM5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
You can see on the product datasheet, PB9903 anti-TRPM5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-08
Question
Is this PB9903 anti-TRPM5 antibody reactive to the isotypes of TRPM5?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The immunogen of PB9903 anti-TRPM5 antibody is E.coli-derived human TRPM5 recombinant protein (Position: S1021-Q1108). Human TRPM5 shares 87.5% amino acid (aa) sequence identity with mouse TRPM5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-11
Question
Is a blocking peptide available for product anti-TRPM5 antibody (PB9903)?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We do provide the blocking peptide for product anti-TRPM5 antibody (PB9903). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-30
Question
Do you have a BSA free version of anti-TRPM5 antibody PB9903 available?
L. Rodriguez
Verified customer
Asked: 2018-06-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TRPM5 antibody PB9903 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-20
