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- Table of Contents
TRPV6 antibody (A02139-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RT4 cells using anti-TRPV6 antibody (A02139-2).
Overlay histogram showing RT4 cells stained with A02139-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV6 Antibody (A02139-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RT4 cells stained with A02139-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TRPV6 Antibody (A02139-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-TRPV6 Antibody Picoband®
TRPV6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
TRPV6 antibody was positively detected by WB in:
human placenta tissue, human PC-3 whole cell, human HepG2 whole cell, human HGC-27 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TRPV6 antibody was positively detected by FC in:
RT4 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Monkey
Predicted
Predicted
Dog
Predicted
Predicted
Product Information
Product Name
Anti-TRPV6 Antibody Picoband®
View all TRPV6 Antibodies
View all TRPV6 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TRPV6 recombinant protein (Position: A15-I765).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
83 kDa
Calculated Molecular Weight
87.3 kDa
Background of TRPV6
TRPV6(Transient Receptor Potential Cation Channel Subfamily V Member 6), also known as ECAC2,CAT1 or CATL, a membrane calcium channel which is responsible for the first step in calcium absorption in the intestine. TRPV6 is a member of the Transient receptor potential(TRP) family of membrane proteins. Using FISH, Peng et al.(2000) mapped the TRPV6 gene to chromosome 7q33-q34. Peng et al.(2000) determined that TRPV6 mediates calcium uptake independent of sodium, chloride, and hydrogen; however, calcium influx was higher at alkaline rather than acidic pH. Functional analysis by Peng et al.(2001) indicated that TRPV5 mediates greater calcium uptake than TRPV6. They concluded that TRPV5 and TRPV6 are calcium-selective channels and share a common ancestral gene.
Cite This Product
Anti-TRPV6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02139-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
90%
High Homology
Mouse
89%
Moderate Homology
Rat
89%
Moderate Homology
Sheep
82%
Moderate Homology
Rabbit
82%
Moderate Homology
Cow
80%
Moderate Homology
Zebrafish
46%
Low homology
Drosophila
28%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TRPV6 antibody
Flow Cytometry (FC) protocol for TRPV6 antibody
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Product Promise
We promise Anti-TRPV6 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A02139-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026