Product Info Summary
SKU: | M02981 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-TRRAP Antibody (C-term)
SKU/Catalog Number
M02981
Size
50 µl
Description
Boster Bio Anti-TRRAP Antibody (C-term) (Catalog # M02981). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TRRAP Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02981)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TRRAP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3015-3049 amino acids from the C-terminal region of human TRRAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02981 is reactive to TRRAP in Human
Applications
M02981 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
437.6kDa
Background of TRRAP
Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. Component of a SWR1- like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling TRRAP with M02981 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
Click image to see more details
All lanes : Anti-TRRAP Antibody (C-term) at 1:2000 dilution
Lane 1: K562 whole cell lysates
Lane 2: Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 438 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Anti-TRRAP Antibody (C-term)at 1:2000 dilution + K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 438 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For TRRAP (Source: Uniprot.org, NCBI)
Gene Name
TRRAP
Full Name
Transformation/transcription domain-associated protein
Weight
437.6kDa
Superfamily
PI3/PI4-kinase family
Alternative Names
350/400 kDa PCAF-associated factor; FLJ10671; PAF350/400; PAF400; PAF400STAF40; STAF40; Tra1 homolog; Tra1; transformation/transcription domain-associated protein; TR-AP; TRRAP TRRAP DEDDFA, DFNA75, PAF350/400, PAF400, STAF40, TR-AP, Tra1 transformation/transcription domain associated protein transformation/transcription domain-associated protein|350/400 kDa PCAF-associated factor|tra1 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRRAP, check out the TRRAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRRAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TRRAP Antibody (C-term) (M02981)
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No publications found for M02981
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