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Product Info Summary
| SKU: | M02981 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-TRRAP Antibody (C-term)
SKU/Catalog Number
M02981
Size
50 µl
Description
Boster Bio Anti-TRRAP Antibody (C-term) (Catalog # M02981). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TRRAP Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02981)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TRRAP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3015-3049 amino acids from the C-terminal region of human TRRAP.
Reactive Species
M02981 is reactive to TRRAP in Human
Calculated molecular weight
437.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02981 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling TRRAP with M02981 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
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All lanes : Anti-TRRAP Antibody (C-term) at 1:2000 dilution
Lane 1: K562 whole cell lysates
Lane 2: Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 438 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-TRRAP Antibody (C-term)at 1:2000 dilution + K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 438 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-TRRAP Antibody (C-term) (M02981)
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