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Product Info Summary
| SKU: | A05961 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Trypsin-3 PRSS3 Antibody
SKU/Catalog Number
A05961
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Trypsin-3 PRSS3 Antibody catalog # A05961. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Trypsin-3 PRSS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05961)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Trypsin-3. AA range:117-166
Reactive Species
A05961 is reactive to PRSS3 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
32.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05961 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Trypsin-3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from A549 cells, using Trypsin-3 antibody.
Specific Publications For Anti-Trypsin-3 PRSS3 Antibody (A05961)
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4 Customer Q&As for Anti-Trypsin-3 PRSS3 Antibody
Question
Can you help my question with product A05961, anti-Trypsin-3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
H. Anderson
Verified customer
Asked: 2020-03-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05961 anti-Trypsin-3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-26
Question
We are currently using anti-Trypsin-3 antibody A05961 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-Trypsin-3 antibody (A05961) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
Question
Will anti-Trypsin-3 antibody A05961 work on primate WB with duodenum?
A. Taylor
Verified customer
Asked: 2018-02-15
Answer
Our lab technicians have not validated anti-Trypsin-3 antibody A05961 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Trypsin-3 antibody A05961 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate duodenum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-15
Question
Do you have a BSA free version of anti-Trypsin-3 antibody A05961 available?
N. Jones
Verified customer
Asked: 2013-04-10
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Trypsin-3 antibody A05961 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-10
