Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)

Reviews: 1
SKU MA1099
Size 100μg/vial
Reactivity Human, Rabbit, Rat
Clonality Monoclonal
Clone Number WH-3
Host Mouse
Ig Isotype Mouse IgG3
Applications WB

Overview

Product Name Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)
SKU/Catalog Number MA1099
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Tryptophan Hydroxylase, tryptophan hydroxylase 1; tryptophan hydroxylase 2 (TPH1; TPH2) detection. Tested with WB in Human;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1099)
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Immunogen Recombinant rabbit tryptophan hydroxylase.
Reactivity Human, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Western blot, 0.5-1μg/ml, Human, rat, rabbit

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

/antibody/ma1099 1 WB anti tryptophan hydroxylase antibody monoclonal wh 3.jpg

Anti-Tryptophan Hydroxylase antibody (monoclonal), MA1099, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate

/m/a/ma1099 2.jpg

Figure 2. Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (MA1099).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tryptophan Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1099) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q8CGU9
Gene Name TPH1; TPH2
Protein Name Tryptophan 5-hydroxylase 2
Tissue Specificity TPH1: Isoform 2 seems to be less widely expressedthan isoform 1.
Alternative Names Tryptophan 5-hydroxylase 2;1.14.16.4;Neuronal tryptophan hydroxylase;Tryptophan 5-monooxygenase 2;Tph2;Ntph;
Molecular Weight 55621 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Research Areas Neuroscience, Neurotransmitter

*You can search these to find other products in these research areas.
Background Tryptophan hydroxylase is the rate-limiting enzyme in the synthesis of serotonin(5-hydroxytryptamine, or 5HT). Tryptophan hydroxylase\atalyzes the biopterin-dependent monooxygenation of tryptophan to5-hydroxytryptophan(5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Human tryptophan hydroxylase(TPH) is mapped to chromosome 11p15.3-p14 by in situ hybridization.

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Order Product (MA1099)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240

USD $240

Ships in 7-10 business days.

Troubleshooting

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Customer Reviews

Review by
Rating:

Source: Biocompare.com

"Outstanding signal of cell bodies and processes"

--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")

Applications Immunohistochemistry
Sample Wistar rat brain
Detection Fluorescence

"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."

(Posted on 8/23/2016)
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to support@bosterbio.com.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tph antibody, tph1 antibody