Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)
Mouse IgG monoclonal antibody for Tryptophan Hydroxylase, tryptophan hydroxylase 1; tryptophan hydroxylase 2 (TPH1; TPH2) detection. Tested with WB in Human;rat;rabbit. No cross reactivity with other proteins.
|Reactivity||Human, Rabbit, Rat|
|Ig Isotype||Mouse IgG3|
|Product Name||Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)
See all Tph2 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for TPH1; TPH2 detection. Host: Mouse.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. TPH1; TPH2 information: Molecular Weight: 55621 MW; Tissue Specificity: TPH1: Isoform 2 seems to be less widely expressedthan isoform 1.|
|Cite This Product||Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1099)|
|Specificity||Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) (MA1099) reacts with Human, Rabbit, Rat Tph2, in native form and recombinant. Superfamily members of Tph2 are not reactive to MA1099.|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Immunogen||Recombinant rabbit tryptophan hydroxylase.|
|Reactivity||Human, Rabbit, Rat|
Our Boster Quality Guarantee for Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Western blot, 0.5-1μg/ml, Human, rat, rabbit
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) (MA1099).Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
Images And Assay Conditions
Anti-Tryptophan Hydroxylase antibody (monoclonal), MA1099, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Figure 2. Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (MA1099).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tryptophan Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1099) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.
Protein Target Info (Source: Uniprot.org)
|Protein Name||Tryptophan 5-hydroxylase 2|
|Tissue Specificity||TPH1: Isoform 2 seems to be less widely expressedthan isoform 1.|
|Alternative Names||Tryptophan 5-hydroxylase 2;220.127.116.11;Neuronal tryptophan hydroxylase;Tryptophan 5-monooxygenase 2;Tph2;Ntph;|
|Molecular Weight||55621 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Research Areas||Human, Rabbit, Rat
*You can search these to find other products in these research areas.
|Background||Tryptophan hydroxylase is the rate-limiting enzyme in the synthesis of serotonin(5-hydroxytryptamine, or 5HT). Tryptophan hydroxylase\atalyzes the biopterin-dependent monooxygenation of tryptophan to5-hydroxytryptophan(5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Human tryptophan hydroxylase(TPH) is mapped to chromosome 11p15.3-p14 by in situ hybridization.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to tph antibody, tph1 antibody