|Reactivity||Human, Rabbit, Rat|
|Ig Isotype||Mouse IgG3|
|Product Name||Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Tryptophan Hydroxylase, tryptophan hydroxylase 1; tryptophan hydroxylase 2 (TPH1; TPH2) detection. Tested with WB in Human;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Tryptophan Hydroxylase Antibody (Monoclonal, WH-3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1099)|
|Immunogen||Recombinant rabbit tryptophan hydroxylase.|
|Reactivity||Human, Rabbit, Rat|
Assay Dilutions Overview
Western blot, 0.5-1μg/ml, Human, rat, rabbit
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
Images And Assay Conditions
Anti-Tryptophan Hydroxylase antibody (monoclonal), MA1099, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Protein Target Info (Source: Uniprot.org)
|Gene Name||TPH1; TPH2|
|Protein Name||Tryptophan 5-hydroxylase 2|
|Tissue Specificity||TPH1: Isoform 2 seems to be less widely expressedthan isoform 1.|
|Alternative Names||Tryptophan 5-hydroxylase 2;220.127.116.11;Neuronal tryptophan hydroxylase;Tryptophan 5-monooxygenase 2;Tph2;Ntph;|
|Molecular Weight||55621 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Research Areas||Neuroscience, Neurotransmitter
*You can search these to find other products in these research areas.
|Background||Tryptophan hydroxylase is the rate-limiting enzyme in the synthesis of serotonin(5-hydroxytryptamine, or 5HT). Tryptophan hydroxylase\atalyzes the biopterin-dependent monooxygenation of tryptophan to5-hydroxytryptophan(5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Human tryptophan hydroxylase(TPH) is mapped to chromosome 11p15.3-p14 by in situ hybridization.|
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- Review by Unattributed Review*
"Outstanding signal of cell bodies and processes"
--- Unattributed Review* (Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews")
Applications Immunohistochemistry Sample Wistar rat brain Detection Fluorescence
"The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy. Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark. Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500."