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Product Info Summary
| SKU: | A06397-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TSC22 domain family protein 1 TSC22D1 Antibody
SKU/Catalog Number
A06397-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TSC22 domain family protein 1 TSC22D1 Antibody (Catalog# A06397-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSC22 domain family protein 1 TSC22D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06397-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TSC22D1. Immunogen sequence location: 71-120
Reactive Species
A06397-1 is reactive to TSC22D1 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
109.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06397-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of the lysates from HepG2 cells using TSC22D1 antibody.
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Immunofluorescence analysis of HepG2 cells, using TSC22D1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TSC22D1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from mouse liver and rat liver cells, using TSC22D1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TSC22 domain family protein 1 TSC22D1 Antibody (A06397-1)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TSC22 domain family protein 1 TSC22D1 Antibody
Question
My lab would like to test anti-TSC22D1 antibody A06397-1 on mouse endothelial cell for research purposes, then I may be interested in using anti-TSC22D1 antibody A06397-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Lewis
Verified customer
Asked: 2020-03-04
Answer
The products we sell, including anti-TSC22D1 antibody A06397-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-04
Question
Please see the WB image, lot number and protocol we used for endothelial cell using anti-TSC22D1 antibody A06397-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
We are currently using anti-TSC22D1 antibody A06397-1 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The anti-TSC22D1 antibody (A06397-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for endothelial cell using anti-TSC22D1 antibody A06397-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-10
Question
Does anti-TSC22D1 antibody A06397-1 work on primate WB with ovary testis?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
Our lab technicians have not tested anti-TSC22D1 antibody A06397-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-TSC22D1 antibody A06397-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate ovary testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-22
Question
Will anti-TSC22D1 antibody A06397-1 work for WB with endothelial cell?
K. Anderson
Verified customer
Asked: 2015-06-05
Answer
According to the expression profile of endothelial cell, TSC22D1 is highly expressed in endothelial cell. So, it is likely that anti-TSC22D1 antibody A06397-1 will work for WB with endothelial cell.
Boster Scientific Support
Answered: 2015-06-05
