Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody

TSHR antibody

Boster Bio Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody (Catalog # M00576). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M00576
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC

Product Name

Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody

View all TSHR Antibodies

SKU/Catalog Number

M00576

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody (Catalog # M00576). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00576)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM222

Isotype

IgG2a, kappa

Immunogen

Recombinant human TSHR, B-Chain protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M00576 is reactive to TSHR in Human

Applications

M00576 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

86.83kDa

Background of TSHR

Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Grave's disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TSHR (Source: Uniprot.org, NCBI)

Gene Name

TSHR

Full Name

Thyrotropin receptor

Weight

86.83kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

CHNG1; LGR3; LGR3hTSHR-I; MGC75129; seven transmembrane helix receptor; thyroid stimulating hormone receptor; Thyroid-stimulating hormone receptor; thyrotropin receptor; thyrotropin receptor-I, hTSHR-I; TSH R; TSHR; TSH-R TSHR CHNG1, LGR3, hTSHR-I thyroid stimulating hormone receptor thyrotropin receptor|TSH receptor|seven transmembrane helix receptor|thyrotropin receptor-I, hTSHR-I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TSHR, check out the TSHR Infographic

TSHR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TSHR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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15 Customer Q&As for Anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal Antibody

Question

Is a blocking peptide available for product anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody (M00576)?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

We do provide the blocking peptide for product anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody (M00576). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-19

Question

I am looking for to test anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 on human thyroid for research purposes, then I may be interested in using anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

The products we sell, including anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-10

Question

Is this M00576 anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody reactive to the isotypes of TSHR?

J. Krishna

Verified customer

Asked: 2019-10-14

Answer

The immunogen of M00576 anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody is Recombinant human TSHR, B-Chain protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-14

Question

Would anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 work for IHC with thyroid?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

According to the expression profile of thyroid, TSHR is highly expressed in thyroid. So, it is likely that anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 will work for IHC with thyroid.

Boster Scientific Support

Answered: 2019-08-29

Question

We have observed staining in human left lobe of thyroid gland. Any tips? Is anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody supposed to stain left lobe of thyroid gland positively?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

Based on literature left lobe of thyroid gland does express TSHR. Based on Uniprot.org, TSHR is expressed in left lobe of thyroid gland, thyroid, ovarian adenocarcinoma, among other tissues. Regarding which tissues have TSHR expression, here are a few articles citing expression in various tissues:
Ovarian adenocarcinoma, Pubmed ID: 15489334
Thyroid, Pubmed ID: 1445355, 2293030, 2610690, 12508121

Boster Scientific Support

Answered: 2019-08-20

Question

We are currently using anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

R. Kulkarni

Verified customer

Asked: 2019-03-11

Answer

The anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody (M00576) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-11

Question

Does M00576 anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

You can see on the product datasheet, M00576 anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-03-01

Question

Do you have a BSA free version of anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 available?

Verified Customer

Verified customer

Asked: 2019-01-04

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-01-04

Question

I was wanting to use your anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody for IHC for human thyroid on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human thyroid identification?

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

As indicated on the product datasheet, M00576 anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human thyroid in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-21

Question

My question regarding product M00576, anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-04-18

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00576 anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-04-18

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid using anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576. Let me know if you need anything else.

O. Moore

Verified customer

Asked: 2017-03-28

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-03-28

Question

I see that the anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 works with IHC, what is the protocol used to produce the result images on the product page?

P. Banerjee

Verified customer

Asked: 2016-01-21

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-01-21

Question

See attached the WB image, lot number and protocol we used for thyroid using anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576. Please let me know if you require anything else.

C. Lewis

Verified customer

Asked: 2014-10-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-29

Question

Will anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 work on goat IHC with thyroid?

A. Johnson

Verified customer

Asked: 2014-01-21

Answer

Our lab technicians have not validated anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 on goat. You can run a BLAST between goat and the immunogen sequence of anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody M00576 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat thyroid in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-01-21

Question

Our team were content with the WB result of your anti-TSH-Receptor, B-Chain (Thyroid Marker) Monoclonal antibody. However we have seen positive staining in thyroid cell membrane using this antibody. Is that expected? Could you tell me where is TSHR supposed to be expressed?

V. Jones

Verified customer

Asked: 2013-03-04

Answer

From what I have seen in literature, thyroid does express TSHR. Generally TSHR expresses in cell membrane. Regarding which tissues have TSHR expression, here are a few articles citing expression in various tissues:
Ovarian adenocarcinoma, Pubmed ID: 15489334
Thyroid, Pubmed ID: 1445355, 2293030, 2610690, 12508121

Boster Scientific Support

Answered: 2013-03-04

Order DetailsPrice
M00576-100ug

100 μg

$468

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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