Click image to see more details
Product Info Summary
| SKU: | A30513 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-TSH1 Antibody
SKU/Catalog Number
A30513
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TSH1 Antibody (Catalog# A30513). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TSH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30513)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TSH1. Immunogen sequence location: 851-900
Reactive Species
A30513 is reactive to TSHZ1 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
117.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30513 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using TSH1 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TSH1 Antibody (A30513)
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Customer Q&As
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4 Customer Q&As for Anti-TSH1 Antibody
Question
We are currently using anti-TSH1 antibody A30513 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
The anti-TSH1 antibody (A30513) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-11
Question
Will anti-TSH1 antibody A30513 work on horse IHC with brain?
Z. Zhao
Verified customer
Asked: 2015-01-12
Answer
Our lab technicians have not tested anti-TSH1 antibody A30513 on horse. You can run a BLAST between horse and the immunogen sequence of anti-TSH1 antibody A30513 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-12
Question
Is a blocking peptide available for product anti-TSH1 antibody (A30513)?
P. Gonzalez
Verified customer
Asked: 2013-08-08
Answer
We do provide the blocking peptide for product anti-TSH1 antibody (A30513). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-08-08
Question
Would A30513 anti-TSH1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Baker
Verified customer
Asked: 2013-01-30
Answer
It shows on the product datasheet, A30513 anti-TSH1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-30
