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- Table of Contents
TSNAXIP1 antibody (A18904) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of TSNAXIP1 using anti-TSNAXIP1 antibody (A18904).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSNAXIP1 antigen affinity purified polyclonal antibody (Catalog # A18904) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSNAXIP1 at approximately 70 kDa, 50 kDa. The expected band size for TSNAXIP1 is at 77 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSNAXIP1 antigen affinity purified polyclonal antibody (Catalog # A18904) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSNAXIP1 at approximately 70 kDa, 50 kDa. The expected band size for TSNAXIP1 is at 77 kDa.
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Anti-TSNAXIP1 Antibody Picoband®
TSNAXIP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Tested Applications
TSNAXIP1 antibody was positively detected by WB in:
human SH-SY5Y whole cell, rat testis tissue, rat C6 whole cell, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-TSNAXIP1 Antibody Picoband®
View all TSNAXIP1 Antibodies
View all TSNAXIP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TSNAXIP1 recombinant protein (Position: M1-E622).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
70 kDa, 50 kDa
Calculated Molecular Weight
76.8 kDa
Background of TSNAXIP1
Using mouse Trax as bait in a yeast 2-hybrid screen, Bray et al. (2002) cloned Tsnaxip1 from a mouse testis cDNA library. The deduced protein contains 709 amino acids and has a coiled-coil structure. Mouse Tsnaxip1 shares 74% homology with the predicted human protein. Northern blot analysis of mouse tissues detected a 2.8-kb transcript only in testis. Northern blot analysis of Tsnaxip1 in developing germ cells indicated predominantly postmeiotic expression. Fluorescence-tagged Tsnaxip1 transfected into mouse fibroblasts showed a cytoplasmic distribution and concentrated staining around the nucleus. Predicted to be involved in cell differentiation and spermatogenesis. Predicted to be located in perinuclear region of cytoplasm. Predicted to be active in cytoplasm
Cite This Product
Anti-TSNAXIP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18904)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
88%
Moderate Homology
Sheep
86%
Moderate Homology
Rabbit
84%
Moderate Homology
Cow
84%
Moderate Homology
Rat
81%
Moderate Homology
Mouse
79%
Moderate Homology
Zebrafish
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TSNAXIP1 antibody
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Product Promise
We promise Anti-TSNAXIP1 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A18904
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026