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- Table of Contents
FIS1 antibody (A01932-2) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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IF analysis of FIS1 using anti-FIS1 antibody (A01932-2).
FIS1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FIS1 Antibody (A01932-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FIS1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FIS1 Antibody (A01932-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-TTC11/FIS1 Antibody Picoband®
FIS1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
FIS1 antibody was positively detected by WB in:
rat brain tissue, rat spleen tissue, rat kidney tissue, mouse brain tissue, mouse spleen tissue, mouse kidney tissue, mouse NIH-3T3 whole cell, mouse RAW2647 whole cell, human HEK293 whole cell, human A549 whole cell, human Hela whole cell, human Raji whole cell, human Caco-2 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FIS1 antibody was positively detected by IHC in:
human lung cancer tissue, human ovarian cancer tissue, human renal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FIS1 antibody was positively detected by ICC/IF in:
Hela cell
FIS1 antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Tested
Tested
Recommend
Recommend
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TTC11/FIS1 Antibody Picoband®
View all FIS1 Antibodies
View all FIS1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TTC11/FIS1 recombinant protein (Position: M1-D121).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
17 kDa
Calculated Molecular Weight
16.9 kDa
Background of FIS1
Mitochondrial fission 1 protein (FIS1) is a protein that in humans is encoded by the FIS1 gene on chromosome 7. It is mapped to 7q22.1. The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. Its role in mitochondrial fission thus implicates it in the regulation of mitochondrial morphology, the cell cycle, and apoptosis. By extension, the protein is involved in associated diseases, including neurodegenerative diseases and cancers.
Cite This Product
Anti-TTC11/FIS1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01932-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
98%
High Homology
Cow
97%
High Homology
Sheep
97%
High Homology
Rat
96%
High Homology
Mouse
95%
High Homology
Monkey
88%
Moderate Homology
Rabbit
85%
Moderate Homology
Zebrafish
64%
Low homology
Drosophila
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FIS1 antibody
Immunohistochemistry (IHC) protocol for FIS1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FIS1 antibody
Flow Cytometry (FC) protocol for FIS1 antibody
Publications
Application:
Species:
Hyperbaric Oxygen Attenuates Cerebral Ischemia–Reperfusion Injury Through ROS-Dependent Remodeling of Microglial Mitochondrial Dynamics
Published: 2026-07-16
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 42511677
Promoting Drp1-Mediated Mitochondrial Division in Nickel Nanoparticles-Induced Reproductive Toxicity in GC-2 Cells
Published: 2025-12-25
Journal: Nanomaterials
Application: WB
Impact Factor: 4.30
Species: Mouse
PMID: 41511229
Portacaval anastomosis promotes fragmentation of mitochondrial network in the cerebellum of male rats
Published: 2025-09-24
Journal: METABOLIC BRAIN DISEASE
Application: WB,IF
Impact Factor: 3.50
Species: Rat
PMID: 40991132
Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study
Published: 2024-04-17
Journal: Nanomaterials
Application: WB
Impact Factor: 4.40
Species: Mouse
PMID: 38668183
Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control
Published: 2023-05-10
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 37234709
Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway
Published: 2022-09-24
Journal: Nutrients
Application: WB
Impact Factor: 6.71
Species: Mouse
PMID: 36235631
A Role for PGC-1a in the Control of Abnormal Mitochondrial Dynamics in Alzheimer’s Disease
Published: 2022-09-13
Journal: Cells
Application: WB,IHC
Impact Factor: 7.67
Species: Mouse
PMID: 36139423
Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model
Published: 2022-06-08
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 3.61
Species: Rat
PMID: 35690143
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Product Promise
We promise Anti-TTC11/FIS1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01932-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026