Product Info Summary
SKU: | A16794-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-TTC38 Antibody Picoband™
SKU/Catalog Number
A16794-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TTC38 Antibody Picoband™ catalog # A16794-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TTC38 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16794-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TTC38 recombinant protein (Position: E267-Q469).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A16794-1 is reactive to TTC38 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
24145 MW
Background of TTC38
TTC38 (tetratricopeptide repeat domain 38) is a 469 amino acid protein that contains three TPR repeats and belongs to the TTC38 family. The gene that encodes TTC38 consists of over 26,000 bases and maps to 22q13. Housing over 500 genes, chromosome 22 is the second smallest chromosome in the human genome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. In addition, translocations between chromosomes 9 and 22 may lead to the formation of the Philadelphia Chromosome and the subsequent production of the novel fusion protein BCR-Abl, a potent cell proliferation activator found in several types of leukemias.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A16794-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a16794 1 ttc38 primary antibodies wb testing 1 a16794 1 ttc38 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a16794-1-ttc38-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of TTC38 using anti-TTC38 antibody (A16794-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC38 antigen affinity purified polyclonal antibody (Catalog # A16794-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC38 at approximately 53 kDa. The expected band size for TTC38 is at 53 kDa.
![a16794 1 ttc38 primary antibodies if testing 2 a16794 1 ttc38 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a16794-1-ttc38-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of TTC38 using anti-TTC38 antibody (A16794-1).
TTC38 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TTC38 Antibody (A16794-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a16794 1 ttc38 primary antibodies fcm testing 3 a16794 1 ttc38 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a16794-1-ttc38-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of HepG2 cells using anti-TTC38 antibody (A16794-1).
Overlay histogram showing HepG2 cells stained with A16794-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC38 Antibody (A16794-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TTC38 (Source: Uniprot.org, NCBI)
Gene Name
TTC38
Full Name
Tetratricopeptide repeat protein 38
Weight
24145 MW
Superfamily
TTC38 family
Alternative Names
FLJ20699; tetratricopeptide repeat domain 38; tetratricopeptide repeat protein 38; TPR repeat protein 38 TTC38 LL22NC03-5H6.5 tetratricopeptide repeat domain 38 tetratricopeptide repeat protein 38|TPR repeat protein 38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TTC38, check out the TTC38 Infographic
![TTC38 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TTC38: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TTC38 Antibody Picoband™ (A16794-1)
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