Product Info Summary
| SKU: | A30693 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TUBGCP5/Gcp5 Antibody
SKU/Catalog Number
A30693
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TUBGCP5/Gcp5 Antibody (Catalog# A30693). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TUBGCP5/Gcp5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30693)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TUBGCP5. Immunogen sequence location: 741-790
Reactive Species
A30693 is reactive to TUBGCP5 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
118.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30693 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of 293 cells using GCP5 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of various cells using GCP5 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of Mouse-kidney mouse-brain HELA KB SH-SY5Y 293T lysis using GCP5 antibody. Antibody was diluted at 1:2000
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Western blot analysis of lysates from HT-29, Jurkat, and 293 cells, using TUBGCP5 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TUBGCP5/Gcp5 Antibody (A30693)
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-TUBGCP5/Gcp5 Antibody
Question
We are currently using anti-TUBGCP5/Gcp5 antibody A30693 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
A. Anderson
Verified customer
Asked: 2013-06-13
Answer
The anti-TUBGCP5/Gcp5 antibody (A30693) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-06-13

