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Product Info Summary
| SKU: | A30694 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TUBGCP6/Gcp6 Antibody
SKU/Catalog Number
A30694
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TUBGCP6/Gcp6 Antibody (Catalog# A30694). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TUBGCP6/Gcp6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30694)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TUBGCP6. Immunogen sequence location: 741-790
Reactive Species
A30694 is reactive to TUBGCP6 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
200.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30694 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of various cells using GCP6 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of the lysates from HeLa cells using TUBGCP6 antibody.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TUBGCP6 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO cells, using TUBGCP6 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TUBGCP6/Gcp6 Antibody (A30694)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TUBGCP6/Gcp6 Antibody
Question
Is this A30694 anti-TUBGCP6/Gcp6 antibody reactive to the isotypes of TUBGCP6?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The immunogen of A30694 anti-TUBGCP6/Gcp6 antibody is A peptide derived from human TUBGCP6. Immunogen sequence location: 741-790. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-30
Question
you antibody to test anti-TUBGCP6/Gcp6 antibody A30694 on human brain for research purposes, then I may be interested in using anti-TUBGCP6/Gcp6 antibody A30694 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
The products we sell, including anti-TUBGCP6/Gcp6 antibody A30694, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-29
Question
Can you help my question with product A30694, anti-TUBGCP6/Gcp6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30694 anti-TUBGCP6/Gcp6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-26
Question
We are currently using anti-TUBGCP6/Gcp6 antibody A30694 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
The anti-TUBGCP6/Gcp6 antibody (A30694) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-12
