Anti-TUT1/Tutase Antibody

Product Name

Anti-TUT1/Tutase Antibody

View all TUT1 Antibodies

SKU/Catalog Number

A09330

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-TUT1/Tutase Antibody (Catalog # A09330). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TUT1/Tutase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09330)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human TUT1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A09330 is reactive to TUT1 in Human, Mouse

Applications

A09330 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

93.847kDa

Background of TUT1

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.

Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Trippe R.,RNA 12:1494-1504(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from 3T3 cells, using TUT1 antiobdy A09330.

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TUT1 antibody A09330.

Gene/Protein Information For TUT1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.7.19; EC 2.7.7.52; FLJ21850; FLJ22267; FLJ22347; MGC131987; MGC149809; nuclear speckle targeted phosphatidylinositol 4-phosphate 5-kinase type I-alpharegulated-poly(A) polymerase; nuclear speckle-targeted PIPK1A-regulated-poly(A) polymerase; PAP-associated domain-containing 2; PAPD2; poly(A) polymerase associated domain containing 2; RBM21; RNA binding motif protein 21; RNA uridylyltransferase; RNA-binding motif protein 21; RNA-binding protein 21; speckle targeted PIP5K1A-regulated poly(A) polymerase; STARPAP; star-PAP; terminal uridylyl transferase 1, U6 snRNA-specific; TUTase; U6 snRN TUT1 PAPD2, RBM21, STARPAP, TENT1, TUTase, URLC6 terminal uridylyl transferase 1, U6 snRNA-specific speckle targeted PIP5K1A-regulated poly(A) polymerase|PAP-associated domain-containing 2|RNA uridylyltransferase|RNA-binding motif protein 21|RNA-binding protein 21|TUTase 6|U6 snRNA-specific terminal uridylyltransferase 1|U6-TUTase|nuclear speckle targeted phosphatidylinositol 4-phosphate 5-kinase type I-alpha regulated-poly(A) polymerase|nuclear speckle-targeted PIPK1A-regulated-poly(A) polymerase|poly(A) polymerase associated domain containing 2|star-PAP|up-regulated in lung cancer 6

For more info on TUT1, check out the TUT1 Infographic

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In this infographic, you will see the following information for TUT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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